Complete nucleotide sequence and molecular characterization of ViaB region encoding Vi antigen in Salmonella typhi
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منابع مشابه
Complete nucleotide sequence and molecular characterization of ViaB region encoding Vi antigen in Salmonella typhi.
Plasmid pGBM124, which contains a 14-kb Salmonella typhi chromosomal DNA fragment capable of producing the Vi antigen in Escherichia coli HB101 and ViaB-deleted S. typhi GIFU 10007-3, was studied. We determined the complete nucleotide sequence of this fragment and found 11 open reading frames. Mutagenesis, subcloning, and complementation analysis showed that three genes (vipA, vipB, and vipC) a...
متن کاملThe Vi antigen of Salmonella typhi: molecular analysis of the viaB locus.
Strains of Salmonella typhi isolated from the blood of patients with typhoid fever invariably express a capsular polysaccharide, termed the Vi antigen. Vi antigen expression is controlled by two separate chromosomal loci, viaA and viaB. The viaA locus is commonly found in enteric bacteria. In contrast, the viaB locus appears to be specific to Vi-expressing strains of Salmonella and Citrobacter....
متن کاملMolecular Characterization of the viaB Locus Encoding the Biosynthetic Machinery for Vi Capsule Formation in Salmonella Typhi
The Vi capsular polysaccharide (CPS) of Salmonella enterica serovar Typhi, the cause of human typhoid, is important for infectivity and virulence. The Vi biosynthetic machinery is encoded within the viaB locus composed of 10 genes involved in regulation of expression (tviA), polymer synthesis (tviB-tviE), and cell surface localization of the CPS (vexA-vexE). We cloned the viaB locus from S. Typ...
متن کاملMOLECULAR CHARACTERIZATION AND OPTIMIZATION OF VI-CAPSULAR POLYSACCHARIDE OF SALMONELLA TYPHI TY6S PRODUCTION IN BIOREACTOR
The role of Vi-capsular polysaccharide (Vi-CPS) in human immunity against infection caused by Salmonella typhi is well known. The downstream process of purification generally causes depolymerization of Vi-CPS to a nonimmunogenic low molecular weight form. In the present study, a standard strain of Sal. typhi Ty6s was grown under submerge cultural conditions in a pilot-plant scale of 90 Liter fe...
متن کاملmolecular characterization and optimization of vi-capsular polysaccharide of salmonella typhi ty6s production in bioreactor
the role of vi-capsular polysaccharide (vi-cps) in human immunity against infection caused by salmonella typhi is well known. the downstream process of purification generally causes depolymerization of vi-cps to a nonimmunogenic low molecular weight form. in the present study, a standard strain of sal. typhi ty6s was grown under submerge cultural conditions in a pilot-plant scale of 90 liter fe...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1993
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.175.14.4456-4465.1993